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KMID : 0545120070170071208
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 7 p.1208 ~ p.1212
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Repeated Batch Production of Epothilone B by Immobilized Sorangium cellulosum
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Park Sang-Woo
Park Su-Jeong
Han Se-Jong
Lee Jin-Won
Kim Dong-Shik
Kim Ji-Heung
Kim Byung-Woo
Lee Jee-Won
Sim Sang-Jun
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Abstract
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Production of extracellular epothilone B, one of the potent anticancer agents, by free and immobilized Sorangium cellulosum was studied using the repeated batch culture process. The concentration of alginate used in immobilization was directly related to the mass transfer rate of nutrients, mechanical stability, and the epothilone B production yield. With the optimized 3% (w/v) calcium alginate carrier, a prolonged repeated batch culture was investigated for the 5 repeated batches for 24 days. The maximum productivity of epothilone B obtained from the alginate-immobilized cells was 5.03 mg/l/day, which is 3 times higher than that of free cells (1.68 mg/l/day).
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KEYWORD
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Epothilone, immobilization, repeated batch culture, calcium alginate, Sorangium cellulosum
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